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Question: the red light wavelength 630 nm illuminates a cuvette...

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The red light (wavelength = 630 nm) illuminates a cuvette at an incident angle of 30° with respect to the normal. The cuvette is made of glass (refractive index = 1.515) and some protein solution is inside the cuvette. At a specific protein solution, the refractive index of the solution is 1.34.

If the protein concentration is increased, the refractive index of the solution gets higher. What physical parameter (e.g., angular change, distance change) can you measure to detect the increased protein concentration? (This is a technique that scientists use to measure protein concentration.)

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