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Question: to get plate with 100 colonies on it how much...

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To get plate with 100 colonies on it, how much must you dilute a bacterial culture with an O.D.500 of 0.15? Assume that you plate 0.1 ml, and that O.D.500 = 0.5 when there are 2 x 108 bacteria/ml.

Describe how you would carry out a dilution of 4 x 105 fold on a bacterial culture, using a total of no more than 50 ml dilution medium and never pipetting less than 100 ul (0.1 ml) at any step.

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