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Question: use the schematic on the left to work out what...

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Use the schematic on the left to work out what would happen during replication. Use thin lines for RNA and thick red lines for DNA. Please pay attention to the leading and lagging strands. Note that the 5ʹ and 3ʹ here refer to the template strand directionality.

Next, fill out the schematic on the right, drawing what you expect to see in the control test tube after the first round of replication is completed. Use thick red lines for ‘hot’ (radioactive) DNA (and blue for ‘cold’ DNA). (Assume that if no new DNA is generated after the first round, the two strands of the original DNA will re-anneal back together.) The yellow bars represent the origin of replication. Remember to start from the products of the first round in part 2!!!

1. Control reaction 5 5 3 3 5 2. What would happen if you blocked the action of PRIMASE, so that it could no longer synthesize RNA? 5 3 5 3 3 53. What would happen if you blocked the action of DNA POLYMERASE III, so that it could not accomplish 5-3 polymerization? 5 3 5 3 3 4. What would happen if you blocked the action of DNA POLYMERASE I, so that it could not accomplish 5-3 exonuclease reactions? 3 3 3 5. What would happen if you blocked the action of DNA LIGASE? 5 3 5

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