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Question: yesterday you inoculated two separate overnight cultures of escherichia coli...

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Yesterday, you inoculated two separate overnight cultures of Escherichia coli and Staphylococcus aureus, respectively. Today in the morning, you realize that you forgot to label the two cultures. Your lab instructor suggests to compare the two cultures under the microscope and to identify the cultures by carrying out the Gram-stain procedure. Which of the following statements is NOT true?

A. The Alcohol is used during the Gram-staining procedure to stabilize the crystal violet in gram-negative bacteria.

B. B and D are not correct.

C. Gram staining procedure will lead to purple color in gram-positive bacteria due to the thick cell wall.

D. Staphylococcus aureus has a spherical morphology and forms cluster, while Escherichia coli has a rod-shaped morphology. After the Gram staining procedure, Staphylococcus aureus will remain purple while Escherichia coli will appear pink.

E. Both species display spherical morphology and form cluster. After the Gram staining procedure, Staphylococcus aureus will appear pink while Escherichia coli remains purple.

F. B, C, D are not correct.

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