Question: you have isolated two different mutations in the mi gene...
You have isolated two different mutations in the mi gene of phage λ that make tiny plaques. These mutations are called my-1^- and my-2^-. You cross and mi-1^- phage with an mi-2^- phage by coinfecting E.coli with phage of both types so that each cell receives at least one phage of each type. Of the 1000 plaques that result from the cross, only 8 form large plaques while the rest are tiny.
a) please draw a map showing possible crossovers between two phages.
b) what is the distance between the mi-1^- and mi-2^- mutations in map units?
c) the genome of phage λ is 5x10^5 bp in physical length and 200 map units in genetic length. What is the distance between mi-1^- and mi-2^- mutations in base pairs?