Question: you need to prepare 1 l medium following this recipe...
- You need to prepare 1 L medium following this recipe:
Sodium phosphate monobasic (0.1 M)
MgSO4x7H2O (50 mM)
What mass of each component do you need to add up to 1 L of distilled water?
After autoclaving the medium, add glucose to final concentration of 2%.
Note: use 40% stock glucose solution sterilized by filtration.
What volume do you need to use from the glucose stock to obtain final concentration of 2% glucose in your medium?