Question: you need to prepare 1 l medium following this recipe...

1. You need to prepare 1 L medium following this recipe:

NaCl (0.8%)

Sodium phosphate monobasic (0.1 M)

MgSO₄x7H₂O (50 mM)

What mass of each component do you need to add up to 1 L of distilled water?

After autoclaving the medium, add glucose to final concentration of 2%.

Note: use 40% stock glucose solution sterilized by filtration.

What volume do you need to use from the glucose stock to obtain final concentration of 2% glucose in your medium?